The present study was conducted to verify the role of oxidative stress in male primary infertility . To acheive this objective , 100 infertile men of ages 20- 40 years and 52 age matched healthy individuals were enrolled . Serum MDA concentration, CAT and GST activity levels were measured by spectrophotometric methods .Serum FSH, LH, prolactin and testosterone concentrations were determined by the assay principle combines an ELFA. The results revealed significant elevation of MDA and significant decreases of CAT and GST activity levels in infertile patients when compared with control group. The highest level of MDA and the lowest activities of CAT and GST were observed in the group of azoospermia with respect to the other subgroup of the infertile patients. MDA levels were found to be significantly positively correlated ,while CAT and GST activities were observed to be significantly negatively correlated with ages and body mass index of the infertile men . The results suggested that oxidative stress is involved in the pathogenesis of primary male infertility. Such involvement implicates the impairment of spermatogenesis and modulation of the male fertility hormone.