Hepatocellular carcinoma (HCC) is one of the most common lifethreatening malignancies in the world. This cancer generally arises within the boundaries of well-defined causal factors. Nutrition research on the health benefits of substances in plant foods (phytochemicals) has recently advanced to a new stage. The efficacy of limonin in hepatoma cell line (HepG2) and in experimental liver cancer described in the resent study offer the potential for reaching on understanding of anticancer potency. Inhibition of HepG2 cells is likely to be selective apoptotic nature of the significant biologically active natural compound limonin. Besides limonin is very much effective in preventing aflatoxin B1 induced multistage hepatocarcinogenesis possibly through antioxidant and antigenotoxic nature, which was confirmed by various biochemical and molecular events. This holds great promise for future research in human beings. The anticancer properties of limonin should provide useful information for the possible application in cancer prevention and cancer therapy