Human life daily exposed too many different factors, which include among others living with the microorganism. Some are for life necessary and essential, but there are some types of microorganisms that can cause serious and fatal diseases or spoil materials and products. Disinfection and sterilization are essential for ensuring that medical and surgical instruments do not transmit pathogenic microorganisms to patients. So this work deals with the measurement, analysis, and then finding the appropriate physical parameters for analyzing the quality of saturated steam sterilization. This work aims to determine the critical parameters system of steam generator to operate according to the norm CSN. Part of the project is the description of the physical sterilization, saturated steam, methods and sensors for the control of steam sterilization.