Synthesis of self-organized titanium dioxide nanotube layers by an electrochemical anodization of Ti at different conditions(time, voltage, concentration of NH4F in electrolyte with glycerol, conductivity and water content) at room temperature (~25?C) were investigated.In the current study, self-organized, vertically-oriented TiO2 nanotubes were successfully prepared by anodization method of a pure Titanium sheet (99.5%) using anodization cell is designed for first time in Iraq (Homemade) from Teflon material according to our knowledge to produce self-ordered Titanium nanotube in organic based electrolytes (glycerol based electrolyte) an electrolyte solution containing (0.5, 1, 1.5 and 2 wt.% NH4F) then added water (2 and 5Wt.% H2O) to (0.5wt.% NH4F) only with 15V. The range of anodizing time and potential were between 1-4hr. and 5-40V, where Wt.% represent weight percentage. Scanning electron microscopy (SEM), Atomic force microscopy(AFM) and (XRD) X-Ray diffraction were employed to characterize the morphology and structure of the obtained Titania templates,optical interferometer(Fizeau frings) method to tube length.