In view of the increasing number of polycystic ovary syndrome (PCOS) among the Sudanese women, and its sequences, such as infertility. This study was conducted to investigate the biochemical changes occurring in the follicular fluid associated with PCOS, which may help in the diagnosis of this disease. This study was carried in the department of biochemistry, Faculty of Medicine in the National Ribat University, in collaboration with Reproductive Health Care Center (RHCC), Sudan Assisted Reproductive Center (SARC) and Asia Hospital in Omdurman. The study was carried out during the period: July 2004- May 2008. A group of 111 women were enrolled in this study; 55 of them were PCOS cases and 56 of them had normal ovaries. Those with normal ovaries represented the control group. The study included women from different Sudanese states; some of them were housewives, while others were employees. The different paramertes measured in PCOS patients were compared with those measured in control group. Six hormones in the follicular fluid were measured by automated chemical analyzer (Immulite 2000).